WebProtein Purification ... (0.001 nM-1 μM) inhibits binding of full-length STING to the solid support with an apparent dissociation constant (K d) of approximately 1.6 nM. STING agonist-4 (Compound 2) (0-100 μM) is 18-fold more potent than cGAMP (an endogenous STING ligand), with an EC 50 of 53.9 μM. WebAug 11, 2024 · The IFN response to cytosolic DNA was shown to be length-dependent at short DNA lengths. Minimally, 20–40 bp are needed for immune activation, depending on …

Both STING and MAVS fish orthologs contribute to the induction …

WebOct 16, 1995 · (b) Expression and purification of a full-length GST-ICP-0-3H fusion protein We tested the pALEX expression vector system for its utility for selectively purifying full-length proteins by cloning in the sequences encoding aa 1-395 of ICP-0, a herpes simplex virus type-1 transcriptional regulator. WebMar 7, 2013 · Full-length STING purified from 293T cells via affinity chromatography was noted to bind to DNA under relatively high-salt and -detergent conditions (Figure 7C). … golf shoes mens adidas

Structural basis of STING binding with and phosphorylation by TBK1

Webintracellular protein STING, a transmembrane protein usually located on an organelle called the endoplasmic reticulum, is a key regulator ... Writing in Nature, Shang et al.2 (page 389) and Zhang et al.3 (page 394) report full-length struc-tures of STING, including STING in complex with the kinase protein TBK1, which initiates the downstream ... Webdomain is known to be unresponsive to STING agonists and is dominant negative when coexpressed with the full-length STING inside cells, we observed that the recombinant TM-deficient STING protein complexed with cGAMP ... using a recombinant TM domain–deficient STING protein as a high- affinity, stable carrier [dK ~73 nM (22)] for … WebProtein expression and purification. ... The coding sequence for full-length human STING fused with a C-terminal FLAG-tag was cloned into the pcDNA3.1A(+) vector (Invitrogen). … health bloom polyclinic