Hct116 p53+/+

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August undefined, 2024

WebFigure 2 Effect of PNA P-6 on cell viability and apoptosis-related protein expression in HCT116 and HT-29 cells. HCT116 ( p53 -wild type) and HT-29 ( p53 -mutant) cells were treated with 10, 15, and 20 μM PNAP-6 for 48 hours. Notes: ( A) … WebMar 15, 2004 · To elucidate mechanisms of resistance to chemotherapies currently used in the first-line treatment of advanced colorectal cancer, we have developed a panel of HCT116 p53 wild-type (p53 (+/+)) and null (p53 (-/-)) isogenic colorectal cancer cell lines resistant to the antimetabolite 5-fluorouracil (5-FU), topoisomerase I inhibitor irinotecan …

What is the BRCA status of the colon cancer cell line HCT116?

WebFeb 27, 2004 · We have used a lentiviral vector to stably express p53 at a physiological level in p53 knockout HCT116 cells. Cells transduced with wild type p53 responded to … WebApr 13, 2024 · HCT116, HCT116 p53 −/−, and HCT116-5FUR cells were treated with mannose (50 mM) and 5-FU (5 μM) alone or in combination for up to 72 h. Cells treated … black cat guns and ammo

Induction of p53-Dependent Apoptosis by Prostaglandin A

WebMay 1, 2007 · We have previously reported that sensitive wt p53 cancer cell lines such as colon HCT116 and RKO or hepatoma HepG2 are killed through p53-mediated apoptosis. … WebJan 17, 2006 · This analysis was based on a combination of denaturing HPLC mutation screening of all exons of the p53 gene, sequencing the cDNA, and assessing the … black cat hacking group

Gene expression profiles in HCT116 and HT29 cells exposed to …

Characterization of p53 wild-type and null isogenic colorectal …

WebTo demonstrate that the p53-Luc fusion retains p53 function, we utilized HCT116 p53–/– cells (kindly provided by Dr B. Vogelstein). The pZ p53-Luc expression plasmid was stably transfected into HCT116 p53–/– cells and the resulting clones were used for … WebTherefore, HCT116 p53+/+ cells are simply marked as HCT116 except for in Figure 1. Our results confirmed that ES induced apoptosis by reducing the expression of c-Myc and … black cat habitsWebApr 13, 2024 · HCT116 p53 −/− cells were cultured in a DMEM medium (Lonza) supplemented with 10% FBS, 100 U/mL of penicillin-streptomycin, 1 mM of sodium pyruvate, and 1× MEM non-essential amino acid (Sigma-Aldrich). All cells were incubated in a humidified incubator (95% air, 5% CO 2) at 37 °C. 2.3. Drugs and Compounds galliard trousers

"WebKSHV; ORF50; MDM2; Sp1; p53; transcriptional repression 1. Introduction Kaposi’s sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus 8 (HHV8), is implicated as the causative agent of several human malignancies, including Kaposi’s sarcoma (KS), primary effusion lymphoma (PEL), and multicentric Castleman’s disease [ … " - Hct116 p53+/+

Hct116 p53+/+

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HCT116 cells have a mutation in codon 13 of the KRAS proto-oncogene, and are suitable transfection targets for gene therapy research. The cells have an epithelial morphology and can metastasize in xenograft models. When transducted with viral vectors carrying the p53 gene, HCT116 cells remain arrested in the G1 phase. The proliferation of HCT116 colonies was found to be inhibited by 5-Fu/P85 copolymer micelles. Furthermore, it was found that the knockout of MARCH2 limited … WebAug 25, 2009 · The use of p53-specific siRNA in HCT116 (p53 +/+) cells also elicited the same divergent pattern of p53 transcriptional behavior, where Lasp1 mRNA was up …

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WebOct 21, 2024 · Treatment of HCT116 isogenic cell lines with either wild-type ( p53+/+ ), null ( p53−/− ), and mutant ( p53R248W/−) p53 and wild-type ( p21+/+) and null ( p21−/−) p21 with 5-FU showed that... WebHCT116 knockout cells were infected with a blank or a p53-expressing lentivirus; HCT116 parental p53 wt cells were infected with a blank lentivirus. Cells were treated with 0.38 mm 5FU for 9 h, as indicated. An …

WebFeb 9, 2024 · To test this therapy model in vivo, we established HCT116 xenograft tumors and compared p53 levels in response to ionizing radiation alone or in combination with … WebApr 7, 2024 · E-cadherin and vimentin staining of metastatic tumors in the livers ( G) and lungs ( H) with HCT116-sh-DDX21 and HCT116-sh-NC cells. Scale bar stands for 50 μm length. The protein expression...

WebDec 2, 2005 · p53 is the most frequently mutated gene in human cancer (Greenblatt et al, 1994). It exerts tumour-suppressor activity, regulating the cell cycle, programmed cell death and DNA repair. p53 functions are mediated by mechanisms that are transcriptional (Yu et al, 1999) and non-transcriptional (Mihara et al, 2003) dependent. WebMar 22, 2024 · Damage-regulated autophagy modulator 1 (DRAM1) induces autophagy and is necessary for p53-mediated apoptosis. However, the signalling pathways regulated by …

WebMar 24, 2004 · The p53 gene was inactivated in HCT116 p53 −/− cells by homologous recombination. Briefly, two promoterless targeting vectors containing either a geneticin or hygromycin resistance gene in place of genomic p53 sequences were sequentially transfected into HCT116 p53 +/+ cells to disrupt both p53 alleles (12).

WebMar 24, 2024 · PGA 2 may induce p53-dependent apoptosis in which DNA-PK activates p53, and DR5, a transcriptional target of p53, plays a pivotal role in HCT116 cells. In contrast to apoptosis in HCT116 cells, PGA may induce apoptosis in a fashion of less potency, which is independent of p53 and DNA-PK in HCT116 p53-/- cells. galliard websiteWebFigure 1 Exposure of four Hct116 cell lines with (+) and without (-) Chk2 and p53 genes to 1 µM C5M So we can see that the Chk2+ means that it has that gene, and the Chk2- means that we knocked out the gene. There’s not really a difference between those two. And the reason we know that is by looking at the error bars. black cat habitatWebMay 19, 2016 · SIRT1 regulates a wide variety of cellular processes, particularly longevity, metabolic control, genotoxic responses, and autophagy, through deacetylating transcriptional factors p53, Forkhead box protein O1 (FOXO1), FOXO3a, NF-κB, MYC, E2F1 and Hypoxia-Inducible Factor (HIF)-1α/HIF-2α, and DNA repair proteins [ 39 ]. galliard wavensmere middleway limitedWebAug 8, 2014 · We verified by PCR however, the HCT116 p53-/- still express all the p53 isoform (D40p53alpha,beta,gamma), d133p53alpha,beta,gamma and also the … galliard the jaw titanWebJul 20, 2024 · Comparable radiosensitivities of p53 +/+ and p53-/-HCT116 colorectal cells induced by CIR were demonstrated, as well as persistent 53BP1 foci indicated DNA … galliard wavensmereWebIn summary, p53-null HCT116 cells transduced with a lentiviral vector expressing wild type p53 respond to DNA-damaging and … galliard wicksideWebMay 21, 2024 · These included HCT116, HCT116.p53 mutant, RKO, and RKO.p53 -/- lines (all from colon cancers) as well as breast cancer cell lines MCF7 and 1001 (MCF7-p53 mutant clone). HeLa cell line was used as a positive control for epithelial to mesenchymal transition (EMT). black cat haken