Web8. máj 2024 · Recent advances utilizing attP/PhiC31 integrase system has permitted the creation of Drosophila cells in which recombination mediated cassette exchange (RMCE) can be utilized to generate stably integrated transgenic cell lines that contain a single copy of the transgene at the desired locus. WebCh. ATCP 31 Note Prefatory Note: (1) The department of agriculture, trade and consumer protection has authority to regulate fertilizer and pesticide bulk storage under s. 94.645, …
Nucleofection of phiC31 Integrase Protein Mediates Sequence ... - PubMed
WebThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA minicircle obtained from a parental minicircle plasmid as a template in an enzyme-mediated rolling circle amplification (RCA) reaction to generate a product that can be cleaved to provide … WebDuring pUAST/PhiC31 integrase-mediated integration, the unique attP site in the ZH-attP-51D fly line genome recombines with the pUAST attB site in the pUAST-PrP vector forming attR and attL sites ... columbia powder lite jackets for women
Pseudo attP sites in favor of transgene integration and
Weblocus. This requires placing an attP integration site into the fly genome, an attB site into the plas-mid containing the DNA to be integrated into the genome, and a source of the phiC31 Integrase [3–6]. Alternatively, cassette exchange can be done using a pair of attP and attB sites [7]. While many attP landing platforms exist, they are not ... Web1. dec 2024 · Three novel PhiC31 pseudo-attP sites were identified on chromosomes 1, 3 and 6, and their genomic features were analyzed. The characterized platform cell lines are stable, and because of single-copy, site-specific R4 recognition attP site, the cell lines could be retargeted for recombinant protein production and drug discovery applications. WebHere we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane … columbia powder lite weste herren